Current Trends in Biotechnology and Pharmacy | 15 July, 2010, Volume 4, No. 3 | Expression of a bacterial chitinase (ChiB) gene enhances antifungal potential in transgenic Litchi chinensis Sonn. (cv. Bedana)

Expression of a bacterial chitinase (ChiB) gene enhances antifungal potential in transgenic Litchi chinensis Sonn. (cv. Bedana)

Abstract

Transgenic litchi harboring chitinase and gus genes were generated via Agrobacterium mediated transformation of zygotic embryos. Confirmation of the transgenic was done by PCR, RT-PCR, Southern blotting and by Western blot. Functional analysis of the transgenic was also attempted.

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