Abstract

The influence of culture conditions and the effect of environmental factors on the growth and production of bioactive metabolites by Streptomyces cheonanensis VUK-A was the focus of this study. The strain exhibited broad spectrum antimicrobial activity against Grampositive, Gram-negative, unicellular and multicellular fungi. The optimum pH and temperature for bioactive metabolite production were 7 and 30°C respectively. Production of bioactive metabolites by the strain was high in asparagine glucose broth as compared to other media tested. Studies on nutritional factors revealed that highest antimicrobial metabolite production was obtained when lactose and peptone at 1% and 0.25% were used as carbon and nitrogen sources respectively. Ninty six hours of incubation was found to be the optimum for bioactive metabolite production by the strain. As the strain exhibited potent antimicrobial activity, it may be explored for biotechnological purposes.