@article{Ifeanyi_Ejiro_Iruoghene_Linus_Ikechukwu_Chiemeka_2023, title={Properties of Glucose Oxidase produced from a newly isolated strain of Aspergillus niger}, volume={17}, url={https://www.abap.co.in/index.php/home/article/view/872}, DOI={10.5530/ctbp.2023.2.23}, abstractNote={<p style="text-align: justify;">Glucose oxidase (GOx) is a key enzyme used in many industries worldwide. To identify the GO x producer strain, Aspergillus niger was isolated from the Beehive and screened for its glucose oxidase-producing capability. Glucose oxidase was mass-produced by a submerged fermentation system. The activity of glucose ox-idase was ascertained by the continuous spec-trophotometric rate determination method. GOx was purified by (70%) ammonium sulphate pre-cipitation, dialyzed, and gel filtration with 2.82, 3.54, and 3.69U/mg specific activity respective-ly. The enzyme exhibited an optimum pH range of 5.5 and a temperature optimum of 40°C. Ag2+ and Hg2+ had a remarkably inhibitory effect on the partially purified GOx, whereas Ca2+, Zn2+, and Mg2+ do not affect the enzyme activity. Cu2+ and Co2+ have a slight inhibitory effect on GOx. Kinetic characteristics of GOx from Aspergillus niger display Vmax as 40 U/ml, and Km as 0.12 mM. The findings on the properties of GOx ex-hibited optimum conditions for industrial appli-cations</p>}, number={2}, journal={Current Trends in Biotechnology and Pharmacy}, author={Ifeanyi, Okoye and Ejiro, Awhin Prosper and Iruoghene, Onosakponome and Linus, Ezugwu Arinze and Ikechukwu, Ajoh Alfred and Chiemeka, Chilaka Ferdinand}, year={2023}, month={May}, pages={850–856} }