TY - JOUR AU - Reddy Shetty , Prakasham AU - Batchu, Uma Rajeswari AU - Buddanaa, Sudheer Kumar AU - Thotaa, Sree Harshika AU - Pulivarthi, Deepthi AU - Cheemalamarri, Chandrasekhar AU - Banotha, Linga AU - Penna, Suprasanna AU - Poda, Sudhakar PY - 2023/05/22 Y2 - 2024/03/29 TI - Studies on metabolic regulation of Schizosaccharomyces pombe biomass production for glucan yield improvement JF - Current Trends in Biotechnology and Pharmacy JA - CTBP VL - 17 IS - 2 SE - Research Paper DO - 10.5530/ctbp.2023.2.16 UR - https://www.abap.co.in/index.php/home/article/view/711 SP - 773-787 AB - <p><em>Schizosaccharomyces pombe (S.pombe) </em>has been playing a pivotal role in biotech industries as a source of glucans; a biological response modifiers (BRM) and also been studied as a model strain for recombinant proteins in molecular biology. Owing to the presence of &gt; 60% glucans in their cell wall structure, the present research was designed to understand the effect of nutrient regulation on growth of <em>S.pombe</em> to economize the glucan production. The study was performed using two different media; yeast extract with supplements (YES) and yeast extract-peptone-dextrose (YPD) and the YPD medium was noticed to be the best and cost-effective for maximum growth of <em>S.pombe</em>. Thereafter, a sequential optimization studies, starting with one factor at a time (OFAT) methodology followed by two step statistical approach Placket-burmann design (PBD) and Response surface methodology (RSM) improved the biomass yield from 14-34.5 g/L (1.5 fold) at shake flask level. Mass transfer of media components, temperature, dextrose and yeast extract have played significant role in metabolism mediated growth of the <em>S.pombe</em>. The major finding of the present study is non-significance of peptone in enhancing the <em>S.pombe</em> biomass. Validation of the above at bioreactor level with dextrose at 4%, rpm at 200, temperature at 28<sup>o</sup>Cand yeast extract at 2% increased the biomass yield from 34.5 - 52 g/L.</p> ER -