In Vitro Cognitive Enhancement Effects of 3-Hydroxyflavone and its Solid Lipid Nanoparticles in PC12 Cells via MTT Assay and ROS Analysis

Chakali Ayyanna; Sujatha  Kuppusamy; Praveen Kumar  Pasala; Saba  Maanvizhi; Suvarna Jyothi  Kantipudi

doi:10.5530/ctbp.2025.4s.14

Authors

Chakali Ayyanna Associate Professor, Department of Pharmacology, Creative Educational Society’s College of Pharmacy, Chinnatekur, Kurnool, 518218, Andhra Pradesh
Sujatha Kuppusamy Professor, Department of Pharmaceutical Chemistry, Sri Ramachandra Faculty of Pharmacy, SRIHER, Porur, Chennai - 600116, Tamil Nadu
Praveen Kumar Pasala Professor, Department of Pharmacology, Raghavendra Institute of Pharmaceutical Education and Research, Ananthapuramu-515721, Andhra Pradesh
Saba Maanvizhi Professor, Department of Pharmaceutics, Sri Ramachandra Faculty of Pharmacy, SRIHER, Porur, Chennai-600116, Tamil Nadu
Suvarna Jyothi Kantipudi Professor, Department of Psychiatry, Sri Ramachandra Medical College & Research institute, SRIHER, Porur, Chennai, 600116, Tamil Nadu

DOI:

https://doi.org/10.5530/ctbp.2025.4s.14

Keywords:

3Hydroxyflavone, 3HFSLNP’s, PC12 cell lines, MTT assay, ROS analysis, cognitive enhancement

Abstract

Oxidative stress with neuronal apoptosis exhibits a strong correlation with neurodegenerative conditions, including Parkinson's disease and Alzheimer's disease. Because of their antioxidant capabilities, flavonoids in particular 3-Hydroxyflavone (3HF), have demonstrated notable neuroprotective and cognitive-enhancing qualities. This study investigates the cognitive-enhancing effects of 3- Hydroxyflavone (3HF), a flavonoid with antioxidant properties, and its solid lipid nanoparticle formulation (3HFSLNP’s), and using PC12 neuronal cell lines. Due to 3-HF's poor solubility and absorption, it was encapsulated in solid lipid nanoparticles via the solvent evaporation method to improve delivery and effectiveness. The nanoparticles were evaluated for particle dimensions, zeta potential, invitro drug release, and encapsulation efficiency. Using MTT assays and ROS measurements, the study found that both 3HF and 3HFSLNP’s amplified cell viability and reduced oxidative stress in a dose-dependent way. However, 3HFSLNP’s demonstrated significantly superior results, indicating cognitive enhancing effects. The present findings imply that 3HFSLNP’s may serve as an impending neuroprotective agent for neurodegenerative diseases such as PD and AD, although additional in vivo studies are required to verify thesefindings.