Inhibition of Growth MCF-7 Breast Cancer Cell with Umbilical Cord Mesencymal Stem Cells (UCMSCs) Derived Secretome

Abstract

Background: Breast cancer is the most common cancer affecting in women and is ex- pected will be increase by 2040. Conventional therapy with chemotherapy as the most widely used therapy has severe side effects and can develop chemotherapy resistance. Currently, Mesenchymal stem cells (MSCs) derived se- cretome have become an alternative for cancer treatment because it contains various soluble factors, such as cytokines or growth factors, and extracellular vesicles. This study aims to evaluate the anticancer effect of secretome on MCF-7 cancer cells. Methods: MSCs were ex- tracted from the umbilical cord and this superna- tant was collected as secretome. The cytotoxic effect of secretome on MCF-7 was examined at concentrations 25-60% with MTT method and the optimum concentration (IC50) was calculat- ed. Result: Secretome showed significantly re- duced proliferation and had cytotoxic effects on the MCF-7 cell line after 75 h treatment with IC₅₀ 50%. Conclution: Secretome has anticancer ef- fect and could significantly reduce cell viability and proliferation.These findings suggest that secretome is a promising candidate for breast cancer theraphy. Further studies are needed to evaluate the mechanism of action of secretome in inhibit the growth of breast cancer cell.