Chitosan Nanoparticles revealing their chemical characterization – Evaluation of Antioxidant and Antibacterial studies

Abstract

Chitosan is a biopolymer categories derived from the exoskeleton of crustaceans, has received much interest due to its biocompatibility, biodegradability, and functional properties. This study investigates the extraction and characterization of Placuna placenta chitosan, commonly known as the windowpane oyster, and shows its resistance antioxidant and antibacterial activities. Chitosan was extracted through a series of demineralization, deproteinization, and deacetylation processes the synthesis of a high-quality biopolymer. The structural features of chitosan were analyzed by UV-Vis Spectrometer, FTIR analysis. Antioxidant activity was evaluated by DPPH radical scavenging and reducing power assays, which showed greater free radical inhibition compared to standard antioxidants. Additionally, using agar well diffusion, the antibacterial activity of chitosan was assessed against a selection of human clinical pathogens, including Salmonella typhi, Klebsiella pneumoniae, Proteus mirabilis, Escherichia coli, Streptococcus pyogenes, Staphylococcus aureus, Vibrio parahaemolyticus, Salmonella paratyphi, Vibrio cholerae, and Klebsiella oxytoca. MBC and MIC were also assessed in this investigation, and the chitosan demonstrated a satisfactory antibacterial activity result.In study shows that the Placuna placenta is a cost-effective, non-toxic source of material possesses with antibacterial activity as well as antioxidant properties.