Interaction Study of Greenly Synthesized silver Nanoparticles with Bovine Serum Albumin (BSA) Using Spectrophotometric and Voltammetric assays

LAIB  Ibtissam; DJAHRA ALI  Boutlilis

doi:10.5530/ctbp.2023.3.39

Authors

LAIB Ibtissam [1]Department of Cellular and Molecular Biology, Faculty of Natural and Life Sciences of, El OuedUniversity, El Oued 39000, El Oued, Algeria[2]Laboratory of Biology, Environment and Health, Faculty of natural and life sciences, El Oued University, El-Oued 39000, Algeria
DJAHRA ALI Boutlilis [1]Department of Cellular and Molecular Biology, Faculty of Natural and Life Sciences of, El OuedUniversity, El Oued 39000, El Oued, Algeria[2]Laboratory of Biology, Environment and Health, Faculty of natural and life sciences, El Oued University, El-Oued 39000, Algeria

DOI:

https://doi.org/10.5530/ctbp.2023.3.39

Keywords:

Silver nanoparticles, Bovine serum albumin, Cyclic voltammetry, Spectrophotometric, Binding free energy, Binding free constant.

Abstract

Due to the considerable constraints impacting conventional pharmaceutical agents and older formulations, nanotechnology plays a vital role in the realm of medicine and in medication delivery. This study's primary goal is to investigate biologically synthesized silver nanoparticles AgNPs with Bovine Serum Albumin (BSA) interact utilizing Spectrophotometric and Voltammetric assays. According to the results, these silver nanoparticles can ligand with BSA (AgNPs-BSA), and the binding is spontaneous with the involvement of electrostatic interactions, according to the computed negative free energy ΔG° values and a constant K. Moreover, the cyclic voltammetric analysis findings confirmed that the nanoparticles were primarily electrostatically bound to BSA.

Overall, these AgNPs have a promising biological future and could be used in nanomedicine and pharmaceuticals.