Non-Recombinant Mutagenesis of Bacillus mojavensis CUIE1819 for Hyper Production of Lipase and Treatment of Polluted Lakes

Authors

  • Irene Infancy J Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru-560029, Karnataka, INDIA.
  • Erumalla Venkata Nagaraju Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru-560029, Karnataka, INDIA.
  • Vaddi Damodara Reddy Department of Biotechnology, REVA University, Yelahanka, Bangalore-560064, Karnataka, INDIA.
  • Kuppusamy Alagesan Paari Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru-560029, Karnataka, INDIA.

DOI:

https://doi.org/10.5530/ctbp.2022.4.80

Keywords:

Microbial Lipase, Screening, Production, Lipase assay, Oil degradability, Mutagenesis

Abstract

Microorganisms that degrade oil contribute significantly to the bioremediation of polluted lakes. Many microorganisms synthesize lipases, which are commercially significant. In the present study microorganisms producing extracellular lipase were isolated from various polluted lakes in Bangalore by using tributyrin agar. A lipase assay was done to determine the most efficient lipase-producing organism, which was then named Bacillus mojavensis CUIE1819 based on 16srRNA sequencing. After UV irradiation, the selected immobilized organisms were used to treat the lake water samples.

The serially diluted water, soil sample, and direct water samples were placed on

Downloads

Published

14-11-2022

How to Cite

J, I. I., Nagaraju, E. V. ., Reddy, V. D. ., & Paari, K. A. . (2022). Non-Recombinant Mutagenesis of Bacillus mojavensis CUIE1819 for Hyper Production of Lipase and Treatment of Polluted Lakes. Current Trends in Biotechnology and Pharmacy, 16(4), 471–480. https://doi.org/10.5530/ctbp.2022.4.80

Issue

Vol. 16 No. 4 (2022): Current Trends in Biotechnology and Pharmacy

Section

Research Paper