Identification of bio-active compounds and invitro anti-inflammatory evaluation of Couroupita guianensis Aubl. fruits

Abstract

Couroupita guianensis, commonly known as Cannon ball tree, is a well-known medicinal and ornamental plant. The tree is known for its broad spectrum of pharmacological activities for treating various human illnesses. The research study was based on identification of bio-actives and evaluation of the invitro anti-inflammatory activity of cannon ball fruits. The methanolic extract of dried fruits was sequentially extracted with Ethyl acetate and Butanol. The marker compounds viz., Tryptanthrin, Indirubin, Methyl perillate and Azealic acid are identified and validated by LCMS and LCMS/MS. Further, Azelaic acid and Methyl perillate are reported for the first time in Coroupita guianensis fruit extract. Also, the Ethyl acetate sub extract and its column fraction recorded promising invitro anti-inflammatory activity of 64.50% and 81.93% at 200ug/ml against LPS induced IL-6 Cytokine in THP1 cell line respectively. These research findings establish the preliminary invitro data on effectiveness and pharmacological rationale for use of C. guianensis fruit extract as an anti-inflammatory agent.